Lung cancer is leading the worldwide cancer related deaths. It can be divided into to two main types: Small Cell Lung Cancer (SCLC) and Non-Small Cell Lung Cancer (NSCLC), the later accounting for 80% of the cases. Common genetic alterations associated with lung cancer are the loss of tumor suppressors, like p53, LKB1 etc. and activating mutations, overexpression, amplification of oncogenes, such EGFR and K-RAS. In lung tumors, EGFR signals through K-RAS, PI3K and STAT3.
Our group is interested in modelling human diseases in mice. We make use of mice genetically modified to study different pathological process such as liver fibrosis and cancer. Recently we have developed a new mouse model for liver fibrosis and we are currently analyzing. Also, by making use of mouse genetic technology, we have generated a mouse model for breast cancer.
From left: Julian Mohrherr, Emilio Casanova-Hevia and Herwig Moll (University Employee)
About Emilio Casanova
Since 2014 he is Professor for "Transgenic Models in Cancer Research" at the Medical University Vienna.
He is an expert in the generation of genetically modified mice and as of 2016 he has more than 52 PubMed based publications with almost 3.000 citations and an h-index of 22 according to his google scholar profile. He serves continuously as reviewer for internationally reputed journals. In addition to the funding of the LBG and partner institutions towards the LBI-CR, he has been responsible to obtain more than 1.5 M€ of external funding towards his research.
EC was PhD student with Pedro Calvo and Miguel Chinchetru at the Department of Biochemistry and Molecular Biology, University of Leon, Spain. He did post-doctoral research at the German Cancer Research Center in Heidelberg with Günther Schütz and at the Biozentrum in Basel with Bernhardt Bettler. Since 2006 he runs his individual research group at the LBI-CR.
Please follow the link for a full CV of Emilio Casanova.
AKT/PKB is a family of serine-threonine kinases activated in response to growth stimuli downstream of the PI3K /PTEN pathway. The AKT family consists of three members: AKT-1, AKT-2 and AKT-3, and has been implicated in different malignancies, such as glioblastoma, ovarian, gastric, colorectal, prostate and breast tumors. Gene amplification and/or over-activation of the three AKT members have been found in different tumors.
Liver fibrosis constitutes a considerable health problem in the human population. In order to further understand the molecular mechanisms underlying liver fibrogenesis it is mandatory to generate genetic mouse models closely resembling human disease development and progression.
Recombinant protein production in eukaryotic cells is one of the main topics of biotechnology. One of the most critical steps is the development of appropriate vectors to express the protein of interest. Bacterial Artificial Chromosomes (BACs) containing the appropriate locus can be used as expression vectors in the protein production field. In collaboration with Dr. A. Bauer, we have developed a BAC-based vector system that improves the protein production by a factor of 10 when compared to a conventional vector in stable HEK293 cell lines.
Andreas Birbach, Pharmacology department, Medical University of Vienna, Austria. BAC-based transgenic mice.
Harald Esterbauer, Dept. of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, Austria. Generation of Knock-in mice.
Boris Kovacic, Institute for Animal Breeding and Genetics, Veterinary University of Vienna, Austria. BAC-based transgenic mice.